Expression of the keratin 10 related genes in buccal mucous membrane exfoliated cells and changes of liver function in coal-burning arsenism
10.3760/cma.j.issn.1000-4955.2009.02.012
- VernacularTitle:燃煤型砷中毒患者口腔黏膜脱落细胞角蛋白10和相关基因表达及肝功能变化
- Author:
Bing, HAN
;
Qin, YANG
;
Jun, WU
;
Yu-mei, YAO
;
Yong-ping, CHEN
;
Xiao-fei, HE
;
Ming-liang, CHENG
- Publication Type:Journal Article
- Keywords:
Arsenic poisoning;
Mouth mucosa;
Genes;
Keratin-10
- From:Chinese Journal of Endemiology
2009;28(2):162-164
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe expression of the keratin 10 (K10), c-myc and cox2 in buccal mucous membrane exfoliated cells, and changes of liver function of coal-burning arsenism patients, in order to explorate coal-burning arsenic poison circumference biology symbol. Methods The buccal mucous membrane exfoliated cells were collected from both arsenism patients and normal controls. Real-time PCR was employed to detect the changes of K10, c-myc, cox2 genes expression in these cells. Simultaneously, circumference venous blood was extracted and examinated liver function. Results K10 was found obviously overexpressed in arsenism patients(3.60±0.94) compared to control(1.82±0.68), the difference had statistics significance(t=2.15, P<0.05), c-mye and cox2 did not found obviously changed(c-myc: 3.50±2.77,3.39±2.07; cox2:5.90±1.40,4.73±1.91; t=1.26,1.65, P> 0.05). The serum ALT of patients(25.83±2A5) obviously increased than control(36.86±1735, t=2.55, P<0.05). Conclusions Expression of K10 gene in buccal mucous membrane cells may be regarded as sensitive molecular markers for skin pathologic changes in arsenic patients. The liver is a sensitive target organ of inorganic arsenic.
