Effect of fluoride on expression of runx2 mRNA and protein in bone tissue of rats
10.3760/cma.j.issn.1000-4955.2010.05.007
- VernacularTitle:氟对大鼠骨组织Runx2 mRNA及其蛋白表达的影响
- Author:
Mei, MEI
;
Yan-ni, YU
;
Bing, GUO
- Publication Type:Journal Article
- Keywords:
Fluorides;
Genes;
Proteins;
Runx2
- From:Chinese Journal of Endemiology
2010;29(5):493-495
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of fluoride on expression of Runx2 mRNA and protein in bone tissue of rats. Methods Fourteen SD rats were randomly divided into two groups: control group(tap water with fluoride ＜ 0.06 mg/L), and fluorosis group(fluoride 50 mg/L in water). After 4 moths, expressions of both mRNA and protein of Runx2 in rat bone tissue were determined by RT-PCR and Western blotting. Results The results showed that the expression of Runx2 mRNA and protein in fluoride-treated bone tissue were 2.287 ± 0.261 and 0.929 ± 0.229, respectively, both of which were significantly higher than those of control group(0.995 ± 0.123,0.317 ± 0.068, t = 11.85,6.78, P ＜ 0.05). Conclusions Fluoride can increase the expression of Runx2 mRNA and protein in bone tissue of rats, and Runx2 may be involved in the pathogenesis of bone injury caused by fluoride.
