Immunodiagnostic value of recombinant membrane protein Tetraspanin 2 of Schistosoma japonicum
10.3760/cma.j.issn.1000-4955.2010.06.010
- VernacularTitle:日本血吸虫重组表膜蛋白Tetraspanin 2免疫诊断价值初探
- Author:
Yun-xia, ZHANG
;
Zhi-rong, ZHAO
- Publication Type:Journal Article
- Keywords:
Schistosoma japonicum;
Membrane proteins;
Immunologic tests;
Enzyme-linked immunosorbent assay
- From:Chinese Journal of Endemiology
2010;29(6):627-630
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the immunodiagnostic potential of recombinant membrane protein Tetraspanin 2 of Schistosoma japonicum(rSjTsp2) as a diagnostic antigen. Methods The rSjTsp2 was expressed and purified as an antigen used in indirect ELISA to detect specific serum antibody against Schistosoma japonicum of rabbits that at different stages of infection, and the results were compared with those of ELISA using natural antigen (AWA) as antigen. Results The sensitivities of rSjTsp2-ELISA were 75.4% (46/61),77.6% (45/58) and 81.5% (44/54), respectively, 2, 4, 6 weeks after rabbits were infected with Schistosoma japonicum, meanwhile the sensitivities of AWA-ELISA were 68.9% (42/61),86.2% (50/58) and 88.9% (48/54), respectively. The sensitivity difference of two methods was not significant statistically (x2 = 0.652, 1.454,1.174, all P ＞ 0.05).Conclusions The rSjTsp2 is expressed successfully in vitro, and rSjTsp2 recombinant protein expressed in prokaryotic system has a certain application potential in immunodiagnosis.
