Oxidative stress-apoptosis mediated STZ-induced diabetic cataract and the interventions of puerarin
10.3980/j.issn.1672-5123.2014.10.08
- VernacularTitle:链脲佐菌素诱导糖尿病性大鼠白内障的氧化应激-细胞凋亡机制及葛根素的改善作用
- Author:
Li, WAN
;
Wen-Bin, LIU
;
Ye-Yu, SHEN
;
Qiu-Li, YU
;
Jing-Jing, ZHANG
- Publication Type:Journal Article
- Keywords:
streptozotocin;
diabetes;
cataract;
oxidative stress;
apoptosis;
puerarin
- From:
International Eye Science
2014;(10):1773-1775
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To explore the involvement of oxidative stress and apoptosis in the pathogenesis of diabetic cataract induced by Streptozotocin ( STZ) and the interventions of puerarin in order to supply references for clinical treatment.
METHODS:Male SD rats were divided into four groups randomly, control group, diabetic group, apocynin group and puerarin group. The diabetic group were replicated by single injection of STZ (65mg/kg, ip). The expression of p22, p47, p67, Bax/Bcl2, Caspase 3 and P53 proteins were detected by Western Blotting.
RESULTS:The diabetic rats were replicated successfully and the expression of Bcl2 was downregulated while the expression of p22, p47, p67, Bax, Caspase 3 and P53 were upregulated in diabetic group with a significant statistical differences when compared with control group (P<0. 05). Apocynin and prerarin can reverse the abnormal expression of the aforementioned proteins dramatically (P<0. 05).
CONCLUSION: NADPH oxidase mediated oxidative stress and P53, Bax/Bcl2 mediated apoptosis are involved in the pathogenesis of diabetic cataract and puerarin can alleviate cataract greatly by inhibiting the aforementioned signal pathway.
