Effect of Daidzein intravitreal injection on optic nerve injury in rats
10.3980/j.issn.1672-5123.2014.08.03
- VernacularTitle:大豆黄酮玻璃体腔注射对大鼠视神经损伤的影响
- Author:
Zhong-Jun, TANG
;
Zhen-Ping, HUANG
;
Wen-Jing, YANG
;
Yong-Xiang, ZOU
;
Ji-Ping, CAI
- Publication Type:Journal Article
- Keywords:
Daidzein;
optic nerve damage;
Caspase-3;
GAP-43
- From:
International Eye Science
2014;(8):1378-1381
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To discuss Daidzein intravitreal injection whether has protective and recovery effects on acute nerve damages.
METHODS:After the crush models of acute optic nerve were set up, 72 males SD rats were divided into 4 groups randomly as common group without surgery, FBS negative control group, Daidzein treatment group ( 10μmol/L, 100μmol/L, 1000μmol/L ) and positive control group using rats nerve growth factor ( mNGF, 100ng/mL ). Three days after interference, all experimental animals were executed. HE staining was used to evaluate morphologic change of the retina, immunohisochemical staining and western-blot tests for identifying and quantifying the distinct expression of Caspase-3 and GAP-43 among the groups.
RESULTS: Compared with the normal group and negative control group, retinal morphology of different concentrations of each Daidzein treatment group and positive control group was more complete, the expression of Caspase-3 protein was relatively lower, the expression of GAP-43 protein was relatively higher, the differences have statistically significance (P<0. 05).CONCLUSION: Daizein injection in the vitreous cavity has the capacity of protection and restoration in rat's acute nerve damages.
