Comparison of endostatin (endostar) and avastin's inhibition effect on mice choroidal neovascularization
10.3980/j.issn.1672-5123.2014.06.04
- VernacularTitle:内皮抑素和 avastin 对小鼠脉络膜新生血管抑制作用的比较观察
- Author:
Jing, LI
;
Yong, MA
;
Hong-Mei, WANG
;
An-Ming, XIE
;
Xuan, LIU
- Publication Type:Journal Article
- Keywords:
choroidal;
neovascularization;
endostatin;
avastin
- From:
International Eye Science
2014;(6):996-999
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To observe the inhibition effect of endostatin ( endostar ) on mice choroidal neovascularization model ( CNV) and compare with the Avastin.
METHODS: Using 532nm laser photocoagulation to establish a mouse model of CNV. We observed the formation of CNV by histopathological examination after 2wk later. Forty successful models of mice were randomly divided into control group (group 1, 10 rats), normal saline group ( group 2, 10 rats ), endostatin group (group 3, 10 rats) and avastin group (group 4, 10 rats) . The drugs were injected into the mice' vitreous after photocoagulation 2wk later. Then 1wk later, we took the mice eyeballs to perform the HE and immunohistochemical staining to observe. The statistical analysis of ANOVA was done by SPSS 16. 0 and the LSD-t test was used for multiple samples, taking P<0. 05 as the test standards.
RESULTS: Two weeks later, HE histopathological examination was done , light microscope showed large amount of new vessels' formation, the positive rate for CNV was 72. 8%. The blank control group compared with the normal saline group P>0. 05, had no inhibitory effect on CNV;endostatin treated group compared with control group, P<0. 05, had a certain inhibitory effect; avastin group compared with the control group, P<0. 05, had an inhibitory effect on CNV; the LSD-t was performed on Avastin group and endostatin group, P<0. 05, which were statistically significant. We thought that the two drugs have different inhibitory effect on mice' CNV, because xAvastin=26. 90 ,xendostatin=29. 13,xAvastin CONCLUSION: Laser-induced CNV animal models of colored mice C57BL/6J is of short time and high rate establishment and it is an ideal model for CNV study. Endostar has certain inhibitory effect on CNV, and it is likely to become one of the important drugs for CNV-related diseases in the future.
