PCNA, Bcl-2 and TERT expression in epiretinal membrane of rat traumatic proliferative vitreoretinopathy
- VernacularTitle:大鼠外伤性PVR视网膜前膜形成中TERT、PCNA和Bcl-2的变化
- Author:
Li-Ping, XUE
;
Feng-Ying, KANG
;
Shi-Xing, HU
;
Xin-Guo, DENG
;
Shao-Chun, LIN
- Publication Type:Journal Article
- Keywords:
traumatic proliferative vitreoretinopathy;
PCNA;
telomerase;
telomerase catalytic subunit;
Bcl-2;
animal model;
immunohistochemistry
- From:
International Eye Science
2005;5(1):19-22
- CountryChina
- Language:Chinese
-
Abstract:
· AIM: To explore the dynamic expression and correlation among telomerase catalytic subunit (TERT), proliferating cell nuclear antigen ( PCNA) and antiapoptosis protein Bd-2 which relate to cell proliferation in epiretinal membrane of rat traumatic proliferative vitreoretinopathy(PVR).· METHODS: S-P technique was applied for immunohistochemical staining of epiretinal membrane of traumatic PVR with TERT, PCNA and Bcl-2 antibody. HE staining was also carried out. The staining results were analyzed with image analysis system.· RESULTS: The positive rate and average A of PCNA protein were upregulated at first and then down-regulated, with the peak value in 14d Group, which was significantly different from those in 7d Group and 28d Group.The positive rate and average A of TERT and Bcl-2 were also upregulated at first and then down-regulated, with the peak value in 14d Group and 21d Group, which were significantly different from those in 7d Group. There was significant correlation among PCNA, Bcl-2 and TERT protein expression (P≤0.01).· CONCLUSTON: TERT and Bcl-2 take part in the regulation of proliferative cells in epiretinal membrane of traumatic proliferative PVR, with high correlation with the dynamic changes of cell proliferation.
