Sequential changes of HIF-1αprotein and mRNA in hypoxic bovine retinal microvessel endothelial cells
- VernacularTitle:缺氧时视网膜血管内皮细胞缺氧诱导因子-1α及其mRNA的时序性表达
- Author:
Ai-Jun, DENG
;
De-Yong, JIANG
- Publication Type:Journal Article
- Keywords:
HIF-1 α;
retina;
microvessel endothelial cells
- From:
International Eye Science
2005;5(2):225-228
- CountryChina
- Language:Chinese
-
Abstract:
● AIM: To investigate the sequential changes of HIF-1 α Protein and mRNA in hypoxic bovine retinal microvessel endothelial cells.● METHODS: The bovine retinal microvessel endothelial cells were cultured in normoxic and CoCl2-induced hypoxic conditions respectively. Expressions of HIF-1 α Protein were measured with immunohistochemical staining, and RT-PCR was used to determine the HIF-1 α mRNA.● RESULTS: HIF-1 α began to increase 1h after hypoxia,and reached the peak at 4h. After 16h, it declined significantly. Compared with the normoxic group, the expression of HIF-1 α protein in the hypoxic groups had significant difference (P＜0.01), and HIF-1 α mRNA expression was unchanged under hypoxia.● CONCLUSION: HIF-1 α participates in the hypoxic procedures in retinal microvessel endothelial cells, and hypoxia induce time-dependent changes of HIF-1 α protein expression, which is not modulated on the transcription level. Analysis of HIF-1 α expression revealed a temporal and spatial changes with regard to the hyperoxic repression, indicating that HIF-1 may play a major role in the development of retinopathy of prematurity and other ischemic retinal disorders such as diabetic retinopathy.
