A new method of duplication of the chronic intraocular hypertension model: underwater electrocoagulation
- VernacularTitle:制作慢性高眼压动物模型的一种新方法--水下电凝巩膜表面静脉法
- Author:
Hong-Bin, LÜ
;
Yan, LI
;
Yuan-Sheng, YUAN
;
Qing-Li, LUO
- Publication Type:Journal Article
- Keywords:
glaucoma;
intraocular hypertension;
animal model
- From:
International Eye Science
2005;5(6):1090-1092
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To study the experimental method of inducing the chronic intraocular hypertension in rat eyes.METHODS: Twenty Wistar rats were randomly divided into ocular hypertension and Sham control groups. Intraocular pressure (IOP) was raised by electrocoagulating at least 3 episcleral and limbal veins on the right eye of each rat in ocular hypertension group and its contralateral eye was used as control. At 1, 2, 3, 4 and 8wk after the electrocoagulation of the veins, IOP were measured. RESULTS: The treatment of electrocoagulation caused a significant IOP increase of the right eyes over the baseline, over the contralateral eyes, and over the sham control eyes (repeated measures ANOVA, P＜0.001). At 1wk,IOP was (30.12± 5.L8 ) mmHg (1kPa=7.5mmHg), and maintained the high IOP up to 8wk.CONCLUSION: The chronic intraocular hypertension model could be successfully created by electrocoagulating three or more episcleral and limbal veins.
