Expression of transforming growth factor-β type 1 receptor and transforming growth factor type 2 receptor in diabetic rat retina
- VernacularTitle:实时荧光定量PCR检测糖尿病大鼠视网膜中转化生长因子β受体-1和受体-2基因表达
- Author:
Dan, CAO
;
Lin, LIU
;
Wei, SHEN
- Publication Type:Journal Article
- Keywords:
diabetic retinopathy;
transforming growth factor-β;
transforming growth factor-β receptor;
streptozotocin;
Real-Time Quantitative PCR
- From:
International Eye Science
2006;6(2):247-251
- CountryChina
- Language:Chinese
-
Abstract:
· AIM: To quantitatively detect gene expression level of transforming growth factor-β type 1 receptor (Tβ R 1) and transforming growth factor-β type 2 receptor (Tβ R 2) in different stage of diabetic rats' retina. To observe and analyze the effect of transforming growth factor-βreceptors on the retina of rat diabetic animal model.· METHODS: 28 healthy adult Sprague-Dawley rats were chosen and randomly divided into two groups of normal control (CON) and diabetes mellitus (DM). Diabetes was induced by streptozotocin (STZ) intraperitoneal injection.Gene expression was detected quantitatively with real-time fluorescence quantitative reverse transcription polymerase chain reaction (QRT-PCR).· RESULTS: The mRNA level of Tβ R 1 and Tβ R 2 was 0.000493± 0.000133 and 0.000166± 0.000057 at 4wk. The mRNA level of Tβ R 1 and Tβ R 2 was 0.000608± 0.000232 and 0.000113± 0.000049 at 12wk. Tβ R 1 expression was gradually elevated during the progression of diabetic retinopathy, Tβ R 2 expression was up-regulated at 4wk,but down-regulated at 12wk.Tβ R 2) may play important role in the pathogenesis of diabetic retinopathy.
