Effect of locally administered rapamycin and cyclosporin A on penetrating keratoplasty rejection rat model
- VernacularTitle:局部联合应用雷帕霉素及环孢霉素A在大鼠角膜移植排斥反应中的作用
- Author:
Bing, LI
;
Jing, HONG
;
Jie, WEI
;
Li-Yan, ZHAO
- Publication Type:Journal Article
- Keywords:
rats;
keratoplasty;
penetrating;
immunologic rejection;
rapamycin;
cyclosporin A;
ophthalmic solution
- From:
International Eye Science
2006;6(2):271-274
- CountryChina
- Language:Chinese
-
Abstract:
· AIM: To evaluate the combined effect of rapamycin ophthalmic solution and cyclosporin A ophthalmic solution on allogeneic transplantation in a rat model.rats were used as donors. The animals were randomly assigned to 4 groups. Group A: control (ophthalmic solution solvent 100μ L); group B: 2g/L rapamycin ophthalmic solution 100μ L; group C: 10g/L cyclosporin A ophthalmic solution 100μ L; group D: 2g/L rapamycin ophthalmic solution 50μ L and 10g/L cyclosporin A ophthalmic solution 50μ L, 4 times every day. The treatment was started on 2d after operation, and the animals were administered until rejection. The grafts were inspected by slit-lamp microscope and the corneal survival time was recorded. The pathologic changes were measured by light microscope.significantly prolonged compared with the control group (P ＜ 0.01). However, the combined therapy (group D) was significantly superior compared with group B and C (P＜0.05, P＜ 0.01). The histopathological findings showed that the inflammation cells, neovascularity in each treated group were significantly fewer than that in control group at 14d after operation.a double drug regimen with rapamycin ophthalmic solution and cyclosporin A ophthalmic solution for the control of acute corneal allograft rejection. It indicats that the combined therapy produced synergistic effect.
