The role of low concentration of dexamethasone on the rabbit corneal epithelial cell
- VernacularTitle:低浓度地塞米松对兔角膜上皮细胞的影响
- Author:
Bing, LIU
;
Dan, LI
;
En-Pu, WANG
;
Hai-Xia, RU
;
Jun-Jun, LIN
;
Mei, ZHANG
;
Yong-Hua, SUN
- Publication Type:Journal Article
- Keywords:
corneal epithelial cell;
dexamethasone;
rabbit;
immunohistochemistry;
electronic microscopy
- From:
International Eye Science
2006;6(1):1-4
- CountryChina
- Language:Chinese
-
Abstract:
AIM: The study was undertaken to investigate the effort of Dexamethasone (DEX) on cultured rabbit corneal epithelial (RCE) cells and rabbit corneal epithelial wound healing.METHODS: For the in vitro experiments, primary cultures of RCE cells were used. DEX in different concentrations was added to cultured RCE cells. The effects were measured with tetrazolium salt (MTT)method and flow cytometry. For the in vivo wound-healing experiments, a central corneal deepithelialization was created and were treated with 0.1g/L DEX eyedrop randomly explain how randomly. Epithelial wound healing was evaluated clinically and analyzed histopathologically using light microscopy along with immunohistochemical staning and electronic microscopy.RESULTS: Less than 0.1g/L DEX didn't influence survival rate in cell culture conditions by MTT assay. Flow cytometric studies revealed that 0.1g/L DFX had no effect on cellular growth phase in cultured rabbit corneal epithelial cells. The mean time of the epithelial healing was significantly shorter in the DEX-treated group than in the control group at 24h. There were strong proliferative-cell-nuclear-antigen(PCNA) expressions in newly generated epithelial cells of both groups. The Dex-treated group had a more regular architecture of stromal lamella and significantly less inflammatory response than the control group under electronic microscopy.CONCLUSION: Less than 0.1g/L DEX had no inhibiting effect on cultured rabbit corneal epithelial cell growth.0.1g/L DEX eye drops can effectively promote epithelial growth and reduce inflammatory response, which may have useful clinical application at the early stage of corneal wound healing process.
