COX-2 and its inhibitor Celecoxib in corneal neovascularization
- VernacularTitle:环氧合酶-2及其抑制剂对角膜新生血管作用的研究
- Author:
Yuan, DONG
;
Ming-Chang, ZHANG
- Publication Type:Journal Article
- Keywords:
corneal neovascularization;
VEGF;
COX-2;
Celecoxib
- From:
International Eye Science
2006;6(3):523-526
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To observe the expression of COX-2 in rat corneal neovascularization (CNV) and its relationship to CNV, and to explore the inhibition of Celecoxib, a COX-2 inhibitor, to CNV.METHODS: The distribution and quantification of COX-2and VEGF was detected by immunohistochemistry. Expression of COX-2 and VEGF mRNA was quantified by RT-PCR.The difference in protein and mRNA expressions of COX-2and VEGF was analyzed to find the correlation between them.RESULTS: Expression of activated COX-2 and VEGF protein and mRNA in CNV had a dynamic change. VEGF and COX-2co-localized. Compared with the control group, expression of both protein, mRNA of COX-2 and VEGF in experimental group Ⅱ and Ⅲ had significant difference (P＜0.05), indicating the correlation between COX-2 and VEGF, while that in experimental group I had no statistical difference (P＞0.05).CONCLUSION: COX-2 expression was up-regulated in inflammatory CNV. COX-2 modulates the expression of VEGF,playing a very important role in CNV. Celecoxib inhibit COX-2expression so as to hold back the CNV.
