Inhibition effect of c-Met inhibitor on proliferation of lens epithelial cells
- VernacularTitle:C-Met抑制剂对人晶状体上皮细胞增殖的抑制作用
- Author:
Xiao-Ling, AN
;
Wei-Ming, WANG
;
Qing, HAN
;
Jin-Hua, TAO
;
Jin-Song, ZHANG
- Publication Type:Journal Article
- Keywords:
lens epithelial cells;
c-Met;
K252a;
Bcl-2;
Caspase-3
- From:
International Eye Science
2006;6(4):743-744
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To observe the inhibition of c-Met inhibitor on proliferation of lens epithelial cells (LECs).METHODS: Human's LECs were cultured and hepatocyte growth factor (HGF) and K252a were added to second passage of cells supplied with Dulbecco's modified eagle's medium (DMEM). MTT assay was used to examine the proliferation of LECs, and Western-blot was used to detect the expression change of Bcl-2 and Caspase-3.RESULTS: The photodensity (A) of HGF (50nmol/L) + K252a (30nmol/L) was not significantly different from that of DMEM control (P＞0.05). The expression of Bcl-2 and Caspase-3 were not significantly different from that in the control group.CONCLUSION: K252a, the inhibitor of c-Met, can effectively inhibit the proliferation of LECs.
