Inhibition of human pterygium fibroblasts in vitro by vitamin E succinate
- VernacularTitle:维生素E琥珀酸酯抑制人翼状胬肉成纤维细胞生长作用的研究
- Author:
Jing, YUAN
;
Jian-Xiong, YU
- Publication Type:Journal Article
- Keywords:
vitamin E succinate;
ptergium;
fibroblasts;
cell culture
- From:
International Eye Science
2006;6(5):984-987
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To study the antiproliferation of vitamin E succinate (VES) on pterygium fibroblasts in vitro and to find a potential agent for prevention and treatment of primary and recurrence pterygium.METHODS: Primary culture and subculture of pterygium fibroblasts were established in vitro ,and different concentrations of VES (0, 10 and 20mg/L) were added to subcultured fibroblasts, respectively. Influence of VES on the growth curve of fibroblast was observed at day 2, 4 and 7 after treatment of VES. 3- [4,5-Dimethylthiazolzyl]-2,5-Diphenyl Tetrazolium Bromide (MTT) assay at 490nm was used to evaluate the effect of the cells proliferation.RESULTS: The addition of VES to culture caused the marked descent of growth curve in comparison with the control group, and the inhibiting rate of 10 and 20mg/L of VES was 33.2% and 46.7%, 67.9%, and 76.8%, 81.7% at day 2,4 and 7, respectively. VES could obviously inhibit the fibroblast proliferation in dose-dependent manner by MTT assay.CONCLUSION: VES can significantly inhibit the proliferation of pterygium fibroblast in vitro.
