Hydroxyapatite modified titanium promotes superior adhension and proliferation of corneal fibroblast in comparison with pure titanium
- VernacularTitle:经羟基磷灰石处理的纯钛表面有利于角膜成纤维细胞的粘附和增殖
- Author:
Xiao, MA
;
Zhao-Hui, LI
;
Yi-Fei, HUANG
;
Yu-Jie, LU
;
Le-Yun, WANG
;
Jing-Xiang, HUANG
- Publication Type:Journal Article
- Keywords:
hydroxyapatite;
titanium;
keratoprosthesis;
corneal fibroblasts
- From:
International Eye Science
2007;7(1):6-9
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To determine whether hydroxyapatite modified titanium promotes superior adhension and proliferation of rabbit corneal fibroblast in comparison with pure titanium.METHODS: We used bioactive hydroxyapatite to modify titanium surfaces. Fourth passage fibroblasts of rabbit cornea were seeded on hydroxyapatite modified titanium surfaces, pure titanium and glass surfaces. Cell adhension, proliferation and morphology were detected at 24 hours, 48 hours, and 72hours using a acridine orange stain. Further studies of cell morphology were performed using scanning electron microscopy.RESULT: Ceil counts were significantly greater on hydroxyapatite modified titanium surfaces at each time point(P＜0.05).At 24 hours, cell spreading was greater on hydroxyapatite-coated titanium and glass than on the pure titanium. At 72 hours, compared with pure titanium and glass surfaces, the cells on hydroxyapatite modified titanium surfaces had greater spreading area and longer stress fibers.CONCLUSIONS: Hydroxyapatite modified titanium promotes superior adhension and proliferation of rabbit corneal fibroblast in comparison with pure titanium.
