Experimental study on cervical lymph nodes removal enhance allograft survival in alkali-burned cornea
- VernacularTitle:颈淋巴结切除术抑制碱烧伤后角膜移植免疫排斥反应的实验研究
- Author:
Shi-Qi, LING
;
Dao-Man, XIANG
;
Shi-Yi, XIAO
;
Hui, ZHANG
- Publication Type:Journal Article
- Keywords:
alkaline burns;
corneal transplantation;
allograft rejection;
lymphadenectomy
- From:
International Eye Science
2007;7(2):287-290
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To explore the inhibitive effects of cervical lymphadenectomy on kerstoplasy after alkaline burns.METHODS:The Wistar rats' corneas were transplanted into Sprague-Dawley(SD)rats' eyes which were randomly divided into 4 groups:group A(control group);group B,the cervicallymphadenectomy group;group C,corneal transplantation after the alkali burn injury;group D,cervical lymphadenectomy following group C.Out of 6 rats in each group,the cornea of one rat Was used for mawophage immunohistochemistry at day 14 after the transplantstion,and the remaining 5 rats were used for studying corneal immune rejection with a slit lamp.The time when allograft rejection occurred was recorded and mean survival times(MST)were compared among the groups.RESULTS:Compared with the MST of group A(10.40±1.14 days),the MST of group B(46.30±9.46 days)Was significantly longer(P＜0.05).MST of grafts between group C(7.00± 1.58 days)and group D(15.00±3.39 days)was also significant (P＜0.05).At 14th day after the transplantation,there was no CD68 immunoreactivity in the graft of group B,and CD68 proteins were expressed to some extent in the grafts of group A and D.However,in the graft of group C,the expression of proteins Was dramatically up-regulated.CONCLUSION:Cervical lymphadenectomy therapy has a significant effect in preventing corneal allograft rejection in normal and alkali burned oorneai beds.
