Research of the role of aquaporins-1 in corneal endothelial fluid transport
- VernacularTitle:水通道蛋白-1在牛角膜内皮细胞液体转运中作用研究
- Author:
Li, ZHANG
;
Li, WANG
- Publication Type:Journal Article
- Keywords:
corneal endothelium;
p-chloromercuribenzene sulfonate;
aquaporin-1
- From:
International Eye Science
2007;7(3):632-634
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the expression of aquaporins-1 (AQP-1)in cultured bovine corneal endothelial cells and to explore the role of AQP-1 in corneal endothelial fluid transport.METHODS:The bovine corneal cells were cultured in DMEM containing 200mL/L neonate bovine serum. AQP-1 expression in the bovine corneal endothelial cells was detected with immunohistochemistry method before and after treatment of the cells with aquaporin inhibitor, p-chloromercuribenzene sulfonate. The osmotic water permeability was determined bymonitoring volume changes of cultured bovine corneal endothelial cells.RESULTS: AQP-1 expression in the membrane of cultured bovine corneal endothelial cells was revealed by positive staining (in brown color). The reading of osmotic water permeability of the cultured bovine corneal endothelial cells before treatment with p-chloromercuribenzene sulfonate was 0.044±0.005cm/s, which significantly decreased to 0.017 0.003 cm/s(n= 15) after treatment.CONCLUSION: AQP-1 expressed in the membrane of cultured bovine corneal endothelial cells may play an important role in fluid transport of corneal endothelial cells. Alteration of the AQP-1 expression may cause abnormal corneal function and corneal edema.
