The inhibitory effect of triamcinolone acetonide on the proliferation of monkey choroid-retinal endothelial cells in Hypoxia condition
- VernacularTitle:曲安奈德对缺氧条件下恒河猴视网膜血管内皮细胞增殖的抑制作用
- Author:
Jian-Hua, XU
;
Zhe-Li, LIU
;
Ruo-Xi, LI
;
Wei, KONG
;
Wei, ZHANG
- Publication Type:Journal Article
- Keywords:
triamcinolone acetonide;
cell cycle;
hypoxia;
apoptosis
- From:
International Eye Science
2006;6(2):275-278
- CountryChina
- Language:Chinese
-
Abstract:
· AIM: To observe the inhibitory effect of triamcinolone acetonide (TA) on the proliferation of monkey choroidretinal endothelial cells (RF/6A) in hypoxia or normal conditions.retinal endothelial cells of rhesus monkey (RF/6A). The effect of TA on the cellular activity was observed by MTT,the effect on cellular proliferation and apoptosis was detected by flow cytometry (FCM).cycle were reduced and the proportion of cells in G2-M phase was increased under the hypoxia condition .TA had a great effect on the cell cycle of choroid-retinal endothelial cells of rhesus monkey and it induced apoptosis of endothelial cells. It relatively increased the S-phase cells and reduced G2-M phase cells under both normal and hypoxia conditions, which indicates its role in blocking cell cycle from s-phase to G2-M phase and reducing mitosis.RF/6A cells while TA has the opposite effect in both normal and hypoxia conditions. TA can also induce apoptosis of endothelial cells.
