Influence of the fibroblast activity by TGF-β2 antisense oligonucleotide in corneal stroma injury of rabbit
- VernacularTitle:TGF-β2反义寡核苷酸对兔角膜基质创伤修复的影响
- Author:
Jin-Ying, LI
;
Shi-Yi, XIAO
;
Pei, FU
- Publication Type:Journal Article
- Keywords:
TGF-β2;
antisense therapy;
corneal repair;
scarring
- From:
International Eye Science
2006;6(2):291-294
- CountryChina
- Language:Chinese
-
Abstract:
· AIM: To investigate the influence of TGF-β 2 antisense oligonucleotide(ASON) on the differentiation, proliferation of stromal fibroblast in corneal stroma injury.both eyes of 28 rabbits, the right eyes were served as experimental group, corneal incisions were sutured with 8-0 coated vicryl suture carrying TGF-β 2 ASON; the left eyes were served as control group, corneal incision were sutured with common 8-0 coated vicryl suture. Rabbits were killed at 4, 7, 14 and 28d after surgery, stromal fibroblasts were examined by immune histochemistry and electron microscopy.significantly reduced the numbers of cells expressingα -smooth muscle actin (α -SMA) and proliferating cell nuclear antigen (PCNA). The ultrastructure of fibroblast had no significant difference in two groups.differentiation and proliferation of stromal fibroblast in corneal injury. This will be a new method to adjust corneal wound repair.
