Promotion of proliferation and migration of Müller cells by RPE cells in a co-culture system
- VernacularTitle:Transwell小室共培养条件下RPE促进 Müller细胞的增殖和迁移
- Author:
Hong-Mei, MA
;
Xiao-Mei, ZHANG
;
Xiao-Bo, FU
;
Wei-Jun, LI
;
Lan, WU
;
Wei, WANG
- Publication Type:Journal Article
- Keywords:
Müller cells;
retinal pigment epithelium;
co-culture;
hypoxia;
normoxia;
Transwell system
- From:
International Eye Science
2008;8(2):219-222
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the role of retinal pigment epithelium (RPE) in the growth of Müller cells using a co-culture system in vitro . METHODS: Müller cells were cocultured with RPE cells under both normoxic and hypoxic conditions in Transwell chamber culture system. Müller cell proliferation was evaluated by MTT assay. The number of cells which migrate through micropores and stay on the outer bottom side of insert systems were observed and counted. RESULTS: The activities of proliferation and migration of Müller cells when cocultured with RPE cells were significantly higher than those of the Müller cells when cultured alone at all time points under both normoxic and hypoxic conditions. However, for both the coculture and control groups, there is no significant difference between the measurements at 3 and 6 hours. CONCLUSION: Evidence suggests that RPE, when co-cultured with Müller cells, can stimulate migration and proliferation of Müller cells under both hypoxic and normoxic conditions in a time-dependent manner; how-ever, there is no evidence to support the synergetic interaction of RPE and Müller cells co-cultured under hypoxic conditions.
