Analysis of antigen specificity using monoclonal and polyclonal antibodies to Cysticercus cellulosae by enzyme-linked immunoelectrotransfer blot technique.
10.3347/kjp.1987.25.2.159
- Author:
Seung Yull CHO
1
;
Shin Yong KANG
;
Suk Il KIM
Author Information
1. Department of Parasitology, College of Medicine, Chung-Ang University, Seoul 151, Korea.
- Publication Type:Original Article
- MeSH:
parasitolgy-helminth-cestoda;
Taenia solium;
cysticercus;
brain;
enzyme-linked immunoelectrotransfer blot;
Fasciola sp.;
Clonorchis sinensis;
Paragonimus westermani
- From:The Korean Journal of Parasitology
1987;25(2):159-167
- CountryRepublic of Korea
- Language:English
-
Abstract:
To analyse the antigen specificity of patients sera from 24 confirmed neurocysticercosis and a monoclonal antibody, SDS-PAGE using 10-15% linear gradient gel and EITB were done. Cystic fluid, saline extracts of scolex and of whole worm of C. cellulosae, saline extracts of sparganum, hydatid cyst fluid, saline extracts of Fasciola, Clonorchis and Paragonimus were used as antigen. Of protein bands in cystic fluid of C. cellulosae, patient sera reacted frequently to bands of 152, 94, 64, 48, 24, 15, 10 and 7 kDa proteins. To saline extracts of scolex and whole worm of C. cellulosae, patients sera reacted frequently to 94, 64, 52, 39, 34, 15 and 10 kDa bands. Two bands in sparganum extract (130 and 64 kDa) and two bands in hydatid cyst fluid (52 and 27 kDa) were cross-reacting bands with sera from cysticercosis patient. Saline extracts of Fasciola, Clonorchis and Paragonimus did not exhibit cross-reacting bands. Monoclonal antibody to cystic fluid of C. cellulosae was found to react with low molecular weight proteins of 15, 10 and 7 kDa.
