Antioxidant effect of hydralazine on retinal pigment epithelial cells and its potential use in the therapy of age-related macular degeneration
- VernacularTitle:肼屈嗪对视网膜色素上皮细胞的抗氧化作用及其治疗老年黄斑变性的潜在作用
- Author:
Yu-Wen, CHENG
;
George C Y CHIOU
- Publication Type:Journal Article
- Keywords:
age-related macular degeneration (AMD);
reactive oxygen species (ROS);
tert-butyl hydroxyperoxide (t-BHP)
- From:
International Eye Science
2008;8(6):1059-1064
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the antioxidant effect of hydralazine under hypoxia-induced damage on retinal pigment epithelial (ARPE-19) cells and the role of reactive oxygen species (ROS) in this effect.METHODS: Human retinal pigment epithelial (hRPE) cells were used to investigate the effect of hydralazine on oxidative stress, including tert-butyl hydroxyperoxide (t-BHP), H2O2, sodium azide (NaN3), and hypoxia induced cell damage. Cell viability was determined by MTT assay.RESULTS: When ARPE-19 cells were treated with oxidative stress induced by ROS, hydralazine showed concentration-dependent protection against t-BHP, H2O2 and hypoxia induced cell damage but not NaN3. Nitric oxide (NO) was not involved in this effect.CONCLUSION: Hydralazine showed antioxidant potential against oxidative stress induced damage in ARPE-19 cells. These effects might be caused through scavenger of ROS. Thus, hydralazine could be used for the treatment of age-related macular degeneration (AMD).
