Expression of transforming growth factor-β type Ⅰ receptor and transforming growth factor-β type Ⅱ receptor in rat retina
- VernacularTitle:转化生长因子-β受体Ⅰ/Ⅱ在大鼠视网膜中基因表达的定量检测
- Author:
Wei, SHEN
;
Lin, LIU
- Publication Type:Journal Article
- Keywords:
TGF-β receptor;
quantitative reverse transcription polymerase chain reaction (QRT-PCR);
gene expression;
retina
- From:
International Eye Science
2008;8(6):1073-1075
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To quantitatively investigate transforming growth factor-β type Ⅰ receptor (TβRⅠ) and transforming growth factor-β type Ⅱ receptor (TβRⅡ) gene expressions in rat retina.METHODS: Sprague-Dawley rats were chosen in this research. Gene expression was detected quantitatively by reverse transcription polymerase chain reaction (RT-PCR) analysis. RESULTS: The expression level of TβRⅠ and TβRⅡ were 0.00034±0.00013 and 0.0001±0.00005, respectively. The expression level of TβRⅠ was obviously higher than that of TβRⅡ in the rat retina with statistical significance (P<0.01). The ratio of TβRⅠ/TβRⅡ was 3.9±1.7.CONCLUSION: Real time quantitative RT-PCR is an effective method to detect differential expression genes in retina. The change of TβRⅠ/TβRⅡ expression may play an important role in the pathogenesis of retinopathy, which could be further investigated in its significance in the development of proliferation retinopathy.
