Quantitative detection of the expression level of transforming growth factor-β1 and-β2 in rat retina with real time PCR
- VernacularTitle:大鼠视网膜中转化生长因子β1和β2基因表达的定量检测
- Author:
Wei, SHEN
;
Lin, LIU
- Publication Type:Journal Article
- Keywords:
retina;
quantitative reverse transcription polymerase chain reaction (QRT-PCR);
TGF-β;
gene expression
- From:
International Eye Science
2008;8(6):1076-1078
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To quantitatively detect the expression level of transforming growth factor-β1 (TGF-β1) and transforming growth factor-β2 (TGF-β2) genes in the retina of normal rat in order to determine the expression difference of TGF-β1 and TGF-β2 in retina.METHODS: The total RNA was isolated from which the first strand of cDNA was prepared. The mRNA levels of TGF-β1 and TGF-β2 were detected quantitatively by real time polymerase chain reaction (PCR).RESULTS: The mRNA levels of TGF-β1 and TGF-β2 were 0.0008±0.0003 and 0.0378±0.009, respectively. Expression of TGF-β2 was obviously higher than that of TGF-β1 in rat retina with statistical significance (t=12.37, P<0.001). The ratio of TGF-β2/TG-β1 was 55.00±26.61.CONCLUSION: QRT-PCR could specifically and accurately detect gene expression level in rat retina. In retina the TGF-β2 gene was expressed more abundantly than TGF-β1. It is suggested that TGF-β2 play an important role in retina diseases.
