Effects of resveratrol on proliferation of retinal vascular endothelial cells and expression of VEGF
- VernacularTitle:白藜芦醇对视网膜血管内皮细胞增殖及VEGF表达的影响
- Author:
Wen-Lin, LI
;
Li, ZHANG
;
Yue-Li, ZHANG
- Publication Type:Journal Article
- Keywords:
resveratrol;
retinal vascular endothelial cells;
cell proliferation;
vascular endothelial growth factor
- From:
International Eye Science
2008;8(6):1087-1090
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To study the effects of resveratrol on the proliferation of human retinal vascular endothelial cells induced by cobalt chloride-simulated hypoxia in vitro.METHODS: CoCl2 (100μmol/L) was used to simulate hypoxic condition, and human retinal vascular endothelial cells were cultured in vitro as model. The cell proliferation was determined by MTT method; SABC method was employed to test the expression of vascular endothelial growth factor (VEGF); and computer image analyzer was used to process data. The effects of resveratrol on the proliferation of vascular endothelial cells were observed.RESULTS: Resveratrol inhibited the proliferation of human retinal vascular endothelial cells induced by CoCl2 in a dose- and time-dependent manner in vitro, meanwhile VEGF expression in all groups which were administered medicine was down-regulated. Both kinds of inhibitive effects of resveratrol were statistically significant (P<0.01).CONCLUSION: Resveratrol can significantly inhibit the proliferation of human retinal vascular endothelial cells and the expression of VEGF, which may provide a new approach for prevention and treatment of retinal neovascular diseases.
