Human optic nerve head astrocytes culture in vitro:Ⅰ. the primary culture and passage
- VernacularTitle:人眼视神经乳头星形胶质细胞体外培养:Ⅰ.原代培养和传代试验
- Author:
Wei-Jia, DAI
;
Culp-Stewwart SHELLEY
;
Cheng, ANNA
;
Flanagan JOHN
;
Ethier Ross C
- Publication Type:Journal Article
- Keywords:
cell culture;
glaucoma,open angle;
opticnerve head
- From:
International Eye Science
2008;8(7):1311-1314
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To culture astrocytes from human donor eyes in order to understand the function of astrocytes in remodelling events in the glaucomatous optic nerve head (ONH).METHODS: Primary cultures were prepared by explantation of human ONH tissue in order to get astrocytes. Laminar criborsa (LC) cells were prepared concurrently for comparison. Astrocyte cultures could be separated from LC cells by selecting medium.Similar procedures were used for LC.RESULTS: Primary ceils grew from human optic nerve head explants 4-8 weeks after explantation. Astrocytes had different morphologies and growth characteristics from LC cells. Type 1B astrocyte cells could grow in medium without FBS. Purified cultures were obtained by second passage and could be harvested by third to fifth passage, which were prepared to use for further study, including being characterized by positive glial fibrillary acidic protein (GFAP) and neural cell adhesion molecule (NCAM) staining.CONCLUSION: Precise dissection of fragment is the most important step to get clear explants for primary culture. Economic and rapid method could be useful to select cells by different mediums, which will help us to get more purified cells for further study.
