Support of acellular porcine corneal stroma for growth of corneal epithelium and stromal cell in vitro
- VernacularTitle:脱细胞猪角膜基质体外支持角膜上皮和基质细胞的生长
- Author:
Xu-Chu, LIN
;
Yan-Nian, HUI
;
Hao, MENG
;
Yong-Jie, ZHANG
;
Yan, JIN
- Publication Type:Journal Article
- Keywords:
acellular porcine cornea stroma;
cornealepithelium;
corneal stromal cells;
tissue engineering
- From:
International Eye Science
2008;8(7):1293-1295
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To determine whether acellular porcine cornea stroma (APCS) could support the growth of the rabbit corneal cells in vitro.METHODS: APCS was prepared. The rabbit's corneal epithelium and stromal cells were cultured and seeded on, APCS in vitro.The observation of phase contrast photograph and histological examination were performed.RESULTS: Histological examination showed the epithe- lium grew on the scaffold of APCS in 2-3 layers at 10th day. The stromal cells adhered to the surface of the scaffold after 24 hours and invaded into the interlaminar of the material at 5th day.CONCLUSION: These results indicate that APCS can support the growth and proliferation of the corneal epithelium and stromal cells in vitro.
