The role of MMP-9 and TIMP-3 in induction of choroidal neovascularization in a murine model
- VernacularTitle:MMP-9、TIMP-3在小鼠脉络膜新生血管形成中的作用
- Author:
Shi-Sheng, ZHANG
;
Hua-Ping, LIAO
;
Lei, ZHANG
;
Cai-Hong, ZHU
;
Qiong, ZHANG
;
Ling, WANG
;
Kang-Sun, WANG
- Publication Type:Journal Article
- Keywords:
matrix metalloproteinase-9;
tissue inhibitor of metalloproteinase-3;
choroidal neovascularization;
mice
- From:
International Eye Science
2008;8(8):1500-1503
- CountryChina
- Language:Chinese
-
Abstract:
· AIM: To examine the expression of MMP-9 and TIMP-3 mRNA during choroidal neovascularization (CNV) in a murine model and to investigate the role of them in the development of CNV. · METHODS: CNV was induced in C57BL/6J mice by intensive diode laser (810nm) photocoagulation (120mW, 75μm, 0. 1s) of the fundus whereafter eyes were enucleated at 1, 3days, 1, 2, and 4 weeks. The MMP-9 and TIMP-3 mRNA expression were analyzed using in situ hybridization and image analysis system. · RESULTS: Both expression of MMP-9 and TIMP-3 mRNA had dynamic changes. For MMP-9, the expression was 1, 2, 4 wk > 3d > 1d (P < 0.05), whereas TIMP-3 mRNA, 3d, 1, 2, 4 wk>1d (P<0.05). · CONCLUSION: The imbalance between the changes of MMP-9 and TIMP-3 may accelerate the degrading of extracelluar matrix, and then be involved in the pathogenesis of CNV.
