A feasibility study of recombinant adeno-associated virus(rAAV)as a vector for transferring a target gene to retina
- VernacularTitle:重组腺相关病毒作为目标基因转移视网膜的载体的可行性研究
- Author:
Jian-Ming, WANG
;
Ya-Zhi, FAN
;
Na, HUI
;
Lei, XIONG
;
Hai-Xiao, FENG
;
Nai-Xue, SUN
- Publication Type:Journal Article
- Keywords:
recombinant adeno-associated virus:green fluorescent protein gene;
gene transfer;
retina
- From:
International Eye Science
2008;8(9):1740-1742
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To study the feasibility of recombinant adeno-associated virus(rAAV)as a vector to transfer the green fluorescent protein(GFP)gene as a target gene into rabbit retina.METHODS:Intravitreal injection of rAAV-gfp was performed in either eye for each rabbit with the other eye taken as control.At the 3rd,7th,and 14th day after injection,the eyeballs were removed,and the retinas were flat-mounted on glass slides to inspect the retinal fluorescence,respectively.RESULTS:After intravitreal injection of rAAV-gfp,the presence of fluorescent spots in the cytoplasm of retinal cells indicated that GFP gene was efficiently transferred and expressed in the rabbit retina.CONCLUSION:Recombinant adeno-associated virus is a reliable and simple vector for transferring target gene,e.g.,GFP gene,to the retina.
