β1-integrin overexpression induces adhesion and migration of rabbit corneal epithelial cells in vitro
- VernacularTitle:β1整合素过表达促进体外兔角膜上皮细胞黏附和迁移
- Author:
You-Dong, WANG
;
Qi, SUN
;
Jin-Song, ZHANG
- Publication Type:Journal Article
- Keywords:
corneal epithelial cells;
β1-integrin;
adhesion;
migration
- From:
International Eye Science
2008;8(11):2157-2160
- CountryChina
- Language:Chinese
-
Abstract:
·AIM:To investigate the effect of β1-integrin overexpression on the adhesion and migration of rabbit corneal epithelial (RCE) cells.·METHODS:Eukaryotic expression vector encoding β1-integrin-GFP fusion DNA was transfected into RCE cells,and the β1-integrin-GFP fusion gene was examined by RT-PCR and Western blot.The adhesion to Matrigel and the migration of the transfected cells were determined by adhesion and mobility assays.The phosphorylation of focal adhesion kinase (FAK) was examined by Western blot.·RESULTS:The overexpression of β1-integrin-GFP fusion gene by RCE cells was successfully established.β1-integrin transfection significantly promoted the adhesion of RCE cells to Matrigel ( P < 0.05 ).β1-integrin overexpression also promoted the migration ability of RCE cells and induced FAK phosphorylation in them (P < 0.05).·CONCLUSION:These data suggest that overexpression of β1-integrin promotes the adhesion and migration of RCE cells and that the FAK pathway may play an important role in this process.
