Preliminary findings of immunological characteristics in primary human corneal endothelial cells
10.3969/j.issn.1672-5123.2009.07.001
- VernacularTitle:人类角膜内皮细胞免疫学特征的初步发现
- Author:
Fan, WANG
;
Bai-Lin, SONG
;
Hong-Yang, GUO
- Publication Type:Journal Article
- Keywords:
antigen presenting cells;
primary human corneal endothelial cells;
peripheral blood mononuclear cells;
costimulatory pathway;
FACS analysis
- From:
International Eye Science
2009;9(7):1221-1223
- CountryChina
- Language:Chinese
-
Abstract:
·AIM: To evaluate the function of primary human corneal endothelial cells(HCEC) serving as immunological cells. ·METHODS: Expression of HLA-DP, -DQ, -DR, CD40, CD80, and CD86 was determined by immunohistochemical methods. Meanwhile, purified peripheral blood mononuclear cells(PBMC) were cocultured with primary HCEC which were pre-treated with and without γ-IFN respectively; activation of lymphocytes was determined by FACS analysis.·RESULTS: In coculture system, T lymphocyte was activated by primary HCEC, HLA-DP, -DQ, -DR and CD40 expression were increased by γ-IFN induction. Costimulatory molecular CD80 was shown on the endothelial cells. ·CONCLUSION: Primary HCEC were assumed to be involved in the corneal transplantation rejection process as potential antigen presenting cells(APC).
