Inhibitory effect of propylene glycol mannate sulfate on growth of rabbit lens epithelial cells in vitro
10.3969/j.issn.1672-5123.2009.11.002
- VernacularTitle:甘糖酯体外对兔晶状体上皮细胞增殖的抑制作用
- Author:
Jin, HUANG
;
Li-Na, XIE
- Publication Type:Journal Article
- Keywords:
propylene glycol mannate sulfate;
lens epithelial cell;
posterior capsular opacification;
capsular bag model
- From:
International Eye Science
2009;9(11):2050-2053
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigate the inhibitory effect of rabbit lens epithelial cell(RLEC)survival and growth by propylene glycol mannate sulfate(PGMS)on the rabbit capsular bag in vitro.METHODS;Capsular bags were prepared from rabbit eyes after extracapsular cataract extraction(ECCE)and incubated in 0.2,0.4,0.8g/L PGMS in 2,5,10 minutes incubation periods.After treatment,the capsular bags were cultured for 7 days in Dulbecco minimum essential medium(DMEM)supplemented with 50mL/L fetal calf serum(FCS).The specimens were examined with light microscopy and transmission electron microscopy(TEM).Capsular bags without receiving PGMS only served as controls.RESULTS:PGMS inhibited the proliferation of RLEC in the manner of concentration and time dependentment.At the threshold protocol of incubation in PGMS at 0.8g/L for 5 or 10 minutes,proliferative activity of cells were largely arrested and nearly no RLEC was seen on the posterior capsule(P＜0.05).Control group had no effect on structure and proliferative activity of RLEC,and the growth proceeded rapidly so that the posterior capsule were totally covered by a confluent monolayer of cell by the end of 7 days.Under TEM,the cells in the control group were tightly arrayed with clearly defined cellular boundary and structure;while cellular deformity and undefined intracellular structure could be seen in the 0.4g/L and 0.8g/L experimental groups.CONCLUSION:PGMS can effectively inhibit the proliferation of RLEC.
