Effect of Buddleia flavonoids drug-containing plasma on the expression of STAT1 phosphoprotein in lacrimal gland epithelial cells in vitro
10.3969/j.issn.1672-5123.2010.01.002
- VernacularTitle:密蒙花总黄酮含药血浆干预干眼症细胞凋亡模型STAT1磷酸化蛋白表达
- Author:
Fang, WANG
;
Qing-Hua, PENG
;
Xiao-Lei, YAO
;
Quan-Long, WU
;
Dian, LI
- Publication Type:Journal Article
- Keywords:
dry eye;
lacrimal gland epithelial cells;
culture;
STAT1 phosphoprotein;
androgen receptor;
flutamide
- From:
International Eye Science
2010;10(1):5-8
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To explore the effect of Buddleia flavonoids drug-containing plasma and androgen receptor(AR) blocker on the expression of STAT1 phosphoprotein.METHODS: In vitro lacrimal gland epithelial cells were cultivated with H2O2 to establish the dry eye apoptosis state. Blank plasma group, Buddleia officinalis plasma total flavonoids interfere with drug-containing group, and the intervention group of testosterone propionate were set. The expressions of STAT1 phosphoprotein of each group were observed by Western blot and AR blocker flutamide was used to explore the intended androgen effect of Buddleia flavonoids.RESULTS: After the intervention of drug-containing plasma, the expression of STAT1 Phosphoprotein in Buddleja officinalis drug-containing plasma intervention group(0.353±0.494) and testosterone propionate intervention group(0.502±0.036) were enhanced and the differences between the two groups were significant (P<0.01). After using the AR blocker in all groups, the expression of STAT1 phosphoprotein in each group (0.268±0.061,0.283±0.106,0.213±0.071) had no difference.CONCLUSION: Buddleja officinalis drug-containing plasma total flavonoids can promote the expression of STAT1 phosphorylation.
