Effect of ICAM-1 on the rejection of corneal transplantation in rabbits
- VernacularTitle:ICAM-1含量变化与兔角膜移植排斥反应的实验研究
- Author:
Xia, LI
;
Xiao-Wei, GAO
;
Bin, RENG
;
Bao-Jiang, LI
;
Yong, ZHAO
- Publication Type:Journal Article
- Keywords:
intercellular adhesion molecule-1;
penetrating keratoplasty;
graft rejection
- From:
International Eye Science
2007;7(4):887-890
- CountryChina
- Language:Chinese
-
Abstract:
· AIM: To investigate the role of intercellular adhesion molecule-1 (ICAM-1) in the graft rejection of rabbit penetrating keratoplasty (PKP).· METHODS: Sutures were used to produce corneal neovascularization (CNV) on New Zealand white rabbits to make PKP model. Graft mean survival time and rejection index were determined. The levels of sICAM-1 in serum and aqueous humor were dynamically determined by enzyme-linked immunosorbent assay (ELISA).Immunohistochemical technique was used on cornea to get the evidence of expression and distribution of ICAM-1.· RESULTS: Graft mean survival time is (12.4±1.3) days.The mean sICAM-1 levels in aqueous humor and serum in the control animals that did not undergo surgery were (16.6±3.6)ng/L and (95.2±6.3) ng/L respectively. Levels in aqueous humor and serum for the graft rejection group increased postoperatively, continued to increase to (53.9±19.2)ng/Land (378.8±30.6)ng/L, compared to those in the other group (P ＜0.01). ICAM-1 positive cells were found in the graft rejection group.· CONCLUSION: ICAM-1 plays a critical role in initiating and maintaining corneal graft rejection. Sequential determination of serum sICAM-1 after operation may be of value in the prediction and diagnosis of acute rejection.
