Effects of endothelin-1 on the cytoskeleton protein F-actin of human trabecular meshwork cells in vitro
10.3969/j.issn.1672-5123.2010.02.002
- VernacularTitle:内皮素-1体外对人小梁细胞骨架肌动蛋白的影响
- Author:
Yan, XIANG
;
Bin, LI
;
Gui-Gang, LI
;
Rui-Lin, WANG
;
Zhi-Qi, CHEN
;
Ling-Juan, XU
;
Lan, CHEN
;
Hui, SHI
;
Hong, ZHANG
- Publication Type:Journal Article
- Keywords:
trabecular meshwork;
endothelin-1;
cyto-skeleton;
F-actin
- From:
International Eye Science
2010;10(2):209-211
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To observe the effect of endothelin-1(ET-1) on the cytoskeleton protein F-actin of cultured human trabecular meshwork (HTM) cells. METHODS: Cultured HTM cells were randomly divided into four groups: control group(0mol/L), low-dose ET-1(10-9mol/L) treatment group, middle-dose ET-1(10-8 mol/L) treatment group, and high-dose ET-1(10-7 mol/L) treatment group. After treated with ET-1, the expression of cytoskeleton protein F-actin in trabecular meshwork was analyzed with Western-blot and the distribution of F-actin was detected with FITC-Phalloidin probe. RESULTS: ET-1 dose-dependently and significantly increased F-actin in trabecular meshwork cells. The F-actin stress fiber and periphery actin fiber highly increased and manifested mild reorganization after treated with ET-1; and there were much more cell-to-cell and cell-to-extracellular matrix attachments formation in ET-1 treated HTM cells than that in the untreated HTM cells. CONCLUSION: ET-1 promoted the expression of cytoskeleton protein F-actin and induced the trabecular meshwork actin cytoskeleton reorganization.
