Differentiation of human bone marrow-derived mesenchymal stem cells into neural-like cells by co-culture with retinal pigment epithelial cells
10.3969/j.issn.1672-5123.2010.03.002
- VernacularTitle:视网膜色素上皮细胞诱导骨髓间充质干细胞分化为神经样细胞
- Author:
Ling-Ling, YANG
;
Qing-Jun, ZHOU
;
Yao, WANG
;
Yi-Qiang, WANG
- Publication Type:Journal Article
- Keywords:
bone marrow-derived mesenchymal stem cells;
retinal pigment epithelial cells;
neural-like cells;
bovine retinal extract
- From:
International Eye Science
2010;10(3):409-412
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To detect the differentiation effects of retinal cells or extracts on bone marrow-derived mesenchymal stem cells (BMSC).METHODS: Human fetal BMSC were previously labeled by carboxyfluorescein succinimidyl ester (CFSE), and co-cultured with retinal pigment epithelial （RPE） cells which were pre-treated with ultraviolet irradiation at a ratio of 1∶1 to induce the differentiation of BMSC for up to 14 days. In some assays, a retinal extract of bovine retinal extract (BRE) was added to detect the potential effects of retinal component on the differentiation of BMSC. In addition, neuron-specific enolase (NSE), Nestin and Glial fibrillary acidic protein (GFAP) immunostaining were performed to determine the characteristics of BMSC.RESULTS: The results indicated that by co-cultured with RPE cells, fetal BMSC were differentiated into neural-like cells expressing special neuronal markers Nestin, GFAP and NSE. And the expression of these markers was obviously increased by BRE.CONCLUSION: Retina derived cells and extracts can induce the differentiation of BMSC into neural-like cells.
