Effects of flavone on the oxidation-induced injury of retinal pigment epithelium cells

VernacularTitle:黄酮对视网膜色素上皮细胞氧化损伤的保护作用
Author: Pei, ZHUANG ; Yi, SHEN ; George C Y CHIOU
Publication Type:Journal Article
Keywords: flavone; age-related macular degeneration; retinal pigment epithelium; oxidation
From: International Eye Science 2010;10(9):1641-1644
CountryChina
Language:Chinese
Abstract: AIM: To investigate the effect of flavone on oxidation-induced injury in retinal pigment epithelium(RPE) cells. METHODS: In in vivo studies, NaIO3-induced RPE degeneration in rat eyes were treated with 5g/L flavone eye drops 3 times a day for 1 week before and 4 weeks after NaIO3injection. At the end of 2 and 4 weeks, all rats were measured c-wave by electroretinogram(ERG). In in vitro studies,ARPE-19 cells were treated with hypoxia, H2O2, NaN3and t-BHP to induce cell damages. MTT assay was used to measure the viable cells. RESULTS: The ERG c-wave results showed that flavone reversed NaIO3-induced injury at the end of 4 weeks. In vitro results showed flavone reversed the various oxidants-induced injuries in RPE cells.CONCLUSION: Flavone could prevent the RPE from oxidation-induced injury both in vivo and in vitro.