[Ca2+]i homeostasis and caspase-3 gene expression in verapamil-induced retinal pigment epithelium cells apoptosis in vitro
10.3969/j.issn.1672-5123.2011.01.001
- VernacularTitle:视网膜色素上皮细胞体外凋亡过程中钙离子平衡与caspase-3基因表达
- Author:
Dong-Bo, PANG
;
Jing, HONG
- Publication Type:Journal Article
- Keywords:
[Ca2+]i homeostasis;
caspase-3 gene;
Ver-induced RPE cells apoptosis
- From:
International Eye Science
2011;11(1):1-3
- CountryChina
- Language:Chinese
-
Abstract:
(Ver)-induced human retinal pigment epithelium (RPE) cells apoptosis.hours to induce RPE cells apoptosis.The expression of apoptotic effector gene caspase-3 was assessed by reverse transcription polymerase chain reaction (RT-PCR).Single cell was measured using fluorescence indicator Fura-3/AM with MetaFluo4.5/coolsnapfx/IX70 intracellular Ca2+ fluorescence imaging system.RPE cells and it significantly increased after co-cultured with Ver.The fluorescence in resting RPE cells was strong and distributed throughout the cells.The nucleus appeared more fluorescent than the cytoplasm.Calcium fluorescence of RPE cells attenuated after co-cultured with Ver.[Ca2+]i homeostasis might play pivotal roles in Ver-induced RPE cells apoptosis.
