Demethylation treatment of NES1 gene for breast carcinoma xenografted in nude mice
- VernacularTitle:乳腺癌裸鼠移植瘤NES1基因去甲基化治疗
- Author:
sheng-ping, HU
;
biao, LI
;
yi-fan, ZHANG
;
min, ZHANG
- Publication Type:Journal Article
- Keywords:
breast carcinoma;
normal epithelial cell specific-1 gene;
5-aza-2′-deoxycytidine
- From:Journal of Shanghai Jiaotong University(Medical Science)
2006;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the anti-tumor effect of 5-aza-2′-deoxycytidine(5-aza-dC) on breast carcinoma xenografted in nude mice. Methods The model of breast carcinoma xenografted in nude mice was established.Ten mice were randomized into the treatment group(treated with 5-aza-dC) and control group(treated with PBS).The mass of the tumors before and after treatment were measured in the two groups,and the inhibition rate of the tumor was calculated and the growth curve was drawn.Immunohistochemistry and Western blotting were employed to detect the expression of normal epithelial cell specific-1(NES1)gene. Results The inhibition rate of the tumor in the treatment group was 57.44%,which was significantly different from the control group(P
