Effects of lithium chloride on transforming growth factor beta and connective tissue growth factor in cultured human Tenon's capsule fibroblasts
10.3980/j.issn.1672-5123.2017.9.08
- VernacularTitle:氯化锂对人眼Tenon囊成纤维细胞TGF-β及CTGF的影响
- Author:
Su-Su, LU
;
Shan-Shan, LIU
;
Xiao-Jun, FAN
;
Xiao-Xiang, SUN
;
Jiang-Hua, BIAN
;
Ji-Bing, WANG
- Keywords:
transforming growth factor beta;
connective tissue growth factor;
Tenon's capsule;
fibroblasts;
lithium chloride
- From:
International Eye Science
2017;17(9):1639-1642
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To research the effects of lithium chloride on transforming growth factor beta (TGF-β) and connective tissue growth factor (CTGF) in cultured human Tenon capsule fibroblasts (HTFs) and explore its mechanism.METHODS:HTFs were cultured and identified by vimentin staining with immunofluorescence and the morphological characteristics.The experimental group was processed 48h with LiCl in concentration of 80mmol/L, the control group without LiCl.The mRNA expression of TGF-β and CTGF in two groups were analyzed with real-time fluorescent quantitative polymerase chain reaction (real time-qPCR) and the protein expression was detected with Western blot.RESULTS:The cultured HTFs expressed TGF-β and CTGF.The mRNA expression of TGF-β and CTGF significantly decreased compared with the control group(t=20.042, 14.995, P<0.05).the protein expression of TGF-β and CTGF also decreased significantly compared with the control group(t=46.058、12.452, P<0.05)CONCLUSION:The cultured HTFs can express TGF-β and CTGF in mRNA and proteins' level.LiCl can reduce the expression of TGF-β and CTGF both in gene and proteins' level.LiCl has the potential to modulate wound healing for glaucoma filtration surgery.
