Effect of photochemotherapy on expression of gelatases
- VernacularTitle:光化学疗法对内皮细胞明胶酶表达的影响
- Author:
hui, DENG
;
ding-fen, YUAN
;
chun-lin, YAN
;
kang-huang, LIAO
- Publication Type:Journal Article
- Keywords:
photochemotherapy;
angiogenesis;
gelatinases
- From:Journal of Shanghai Jiaotong University(Medical Science)
2006;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determine the effect of photochemotherapy on the expression of gelatinases. Methods Reverse transcription-polymerase chain reaction and gelatine zymography were employed to detect the effect of photochemotherapy on the expression and bioactivity of gelatinases at mRNA and protein levels respectively. Results Combination of ultraviolet A(UVA,0.8-2.0 J/cm2) and 8-methoxypsoralen(8-MOP,100 ng/mL) resulted in a decrease in the expression and bioactivity of gelatinases. Conclusion Photochemotherapy can inhibit the angiogenesis of endothelial cells through downregulating the expression and bioactivity of gelatinases.
