Research on the promoting role of apelin-13 in proliferation, migration and capillary-like tube formation of RF/6A cells
10.3980/j.issn.1672-5123.2017.6.08
- VernacularTitle:Apelin-13体外促进猴视网膜血管内皮细胞增殖和迁移及管腔形成
- Author:
Kun-Peng, XIE
;
Ping, LIU
;
Xin, WANG
;
Jun-Hui, DU
- Keywords:
apelin;
apelin-13;
angiogenesis;
retinal neovascularization
- From:
International Eye Science
2017;17(6):1042-1046
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the effects of apelin-13 on proliferation, migration and capillary-like tube formation of a monkey choroid / retinal endothelial cell line, RF/6A, to clarify whether apelin-13 could promote retinal angiogenesis in vitro.METHODS: RF/6A cells in good conditions were administrated with DMSO (the control group), apelin-13 at 0.1μmol/L (low dose group) or apelin-13 at 1μmol/L (high dose group).Cell proliferation, migration and capillary-like tube formation were detected by using the MTT assay, scratch assay and matrigel assay, respectively, at 24h after plating the cells.RESULTS: Cell proliferation was promoted in both low and high dose apelin-13 groups compared to the control cells (P<0.05);the cell migration distance of both apelin-13 groups was significantly greater than that of the control group (P<0.05);and the number of capillary-like tube structures of both apelin-13 groups was significantly larger than that of the control cells (P<0.05).In addition, cell proliferation, migration and tube formation increased as the concentration of apelin-13 increased.CONCLUSION: Apelin-13 could obviously promote the angiogenesis capacity of RF/6A cells, suggesting that apelin-13 was an important pro-angiogenic factor in retinal endothelial cells.
