Effects of estrogen on the expression of MMP-2, TIMP-2 and TGF-β1 in human corneal stromal cells

VernacularTitle:雌激素对人角膜基质细胞MMP-2、TIMP-2及TGF-β1表达的影响
Author: Yan, TIAN ; Hong-Bo, YIN ; Ying-Ping, DENG
Keywords: 17β-estradiol; corneal stromal cell; matrix metalloproteinases-2; tissue inhibitor of metalloproteinases-2; transforming growth factor-β1
From: International Eye Science 2017;17(7):1232-1236
CountryChina
Language:Chinese
Abstract: AIM:To investigate the effects of estrogen on the expression of matrix metalloproteinases-2(MMP-2), tissue inhibitor of metalloproteinases-2(TIMP-2) and transforming growth factor-β1(TGF-β1) in cultured human corneal stromal cells.METHODS: Inflammatory environments of human corneal stromal cells were simulated by using 1.5ng/mL IL-1β.The cells were then treated with or without different concentrations of estrogen(0, 1×10-4, 1×10-6, 1×10-8, 1×10-10mol/L estradiol)in vitro.Cell viability was evaluated by MTT.Expression levels of MMP-2, TIMP-2 and TGF-β1 proteins were measured by enzyme-linked immunosorbent assay(ELISA).RESULTS:Estrogen did not affect the viability of human corneal stromal cells.Compared with the control group, expression levels of MMP-2 and TGF-β1 proteins in E2 treatment group significantly decreased after being treated with estrogen, while the expression level of TIMP-2 significantly increased.CONCLUSION: Estrogen could, to some extent, down-regulate the expression of MMP-2 and TGF-β1 and up-regulate the expression of TIMP-2, which might contribute to protecting human cornea.