The protection of salidroside on oxidative stress induced in human lens epithelium cells
10.3980/j.issn.1672-5123.2017.10.07
- VernacularTitle:红景天苷对H2O2诱导的人晶状体上皮细胞氧化应激损伤的保护作用
- Author:
Li-Ting, LIU
;
Wen-Tao, ZHENG
;
Ping, LIU
;
Li-Juan, ZHANG
- Keywords:
salidroside;
hydrogen peroxide;
human lens epithelium cell;
protect
- From:
International Eye Science
2017;17(10):1834-1836
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To explore the effect of different concentrations of salidroside on H2 O2 induced oxidative stress damage in human lens epithelium cells ( HLEC) . · METHODS: HLEC were cultured and divided into negative control group: cultured in normal cultivation;oxidative damage group: treated with 100μmol/L H2 O2 for 12h; Salidroside low concentration group: 10μmol/L salidroside treated for 24h and H2 O2 treated for 12h;Salidroside high concentration group: 100μmol/L salidroside treated for 24h and H2 O2 treated for 12h. MTT method was applied to observe the effect of salidroside on HLEC survival rate. Morphological change of each group were observed and recorded under inverted microscope. DCFH-DA fluorescent probe was applied to detect intracellular ROS changes; content of malondialdehyde ( MDA ) , superoxide dismutase ( SOD ) and glutathione peroxidase ( GSH-Px ) in supernatants were detected by pectrophotometer. · RESULTS: Salidroside obviously inhibited H2 O2 -induced HLEC vitality decline, inhibited ROS generation in cells, causing SOD, GSH-Px levels increased and MDA levels decreased. ·CONCLUSION:Salidroside inhibited H2 O2 induced HLEC injury by decreasing the intracellular MDA content levels and increasing SOD, GSH-Px content levels, which conclude that salidroside may have a certain role in the treatment of HLEC damage.
