Analysis of AmpC beta-lactamase gene in Pseudomonas aeruginosa .
- Author:
Ming, NI
;
Dongshen, ZHANG
;
Junying, QI
- Publication Type:Journal Article
- MeSH:
Bacterial Proteins/*genetics;
DNA, Bacterial/genetics;
Point Mutation/*genetics;
Pseudomonas aeruginosa/enzymology;
Pseudomonas aeruginosa/*genetics;
Sequence Analysis, DNA;
beta-Lactam Resistance/*genetics;
beta-Lactamases/*genetics
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2005;25(1):17-9, 23
- CountryChina
- Language:English
-
Abstract:
The gene and the amino acid sequence of the structural and regulatory region of the Pseudomonas aeruginosa with different resistance patterns were analyzed. Six strains with different resistance patterns were selected and the AmpC beta-lactamase was identified. The objective gene fragment was amplified by colonies PCR. The sequences of the PCR-products were analyzed. The DNA sequence of the structural gene ampC and the regulatory genes ampR, ampD and ampE was detected. The 6 strains and the wild-type Pseudomonas aeruginosa are highly homogeneous in structural and regulatory region. Some new mutant points were found.
