Cell attachment of periodontal ligament cells on commercially pure titanium at the early stage.
- Author:
Bin, ZHOU
;
Yingguang, CAO
;
Lijuan, WU
;
Yanxiang, YUAN
;
Yinping, ZENG
- Publication Type:Journal Article
- MeSH:
Biocompatible Materials/pharmacology;
Cell Adhesion;
Cell-Matrix Junctions;
Cells, Cultured;
*Dental Implants;
Periodontal Ligament/*cytology;
Surface Properties;
Time Factors;
Titanium/*pharmacology
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2004;24(3):307-8, 310
- CountryChina
- Language:English
-
Abstract:
In order to study the character of periodontal ligament cells (PDLCs) attaching on commercially pure titanium (cpTi) by morphology and metrology on the early stage (24 h), 1 x 10(5)/ml PDLCs in 2 ml culture medium were seeded on cpTi discs fixed in 24-well culture plates. Morphology of cell attachment was observed by contrast phase microscope, scanning electron microscope (SEM) and fluroscence microscopy. Cell adhesion was analyzed by MTT at 0.5, 1, 2, 4 h respectively. PDLCs could attach and spread on cpTi discs. SEM showed that PDLCs had pseudopod-like protuberance. PDLCs showed different attaching phases and reached saturation in cell number at 2 h. It was concluded that PDLCs had good biocompatibility with cpTi, and showed a regular and dynamic pattern in the process of attaching to cpTi.
