Effects of different dialysates on apoptosis and expression of PKC? of U937 cell line
- VernacularTitle:不同血液透析液对U937细胞凋亡和PKC?表达的影响
- Author:
xiao-juan, ZHU
;
li-li, GUO
;
yu, PAN
;
long-yi, TAN
;
bi-gu, ZHANG
;
yan, JIN
;
ming-zhu, HUANG
;
guo-lan, ZHANG
;
hai-yan, MENG
;
wei-ya, BO
;
qin-jun, XU
;
hui-min, JIN
- Publication Type:Journal Article
- Keywords:
dialysate;
U937 cell;
cell apoptosis;
protein kinase C-?
- From:Journal of Shanghai Jiaotong University(Medical Science)
2006;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of different dialysates on expression of protein kinase C-? (PKC?) and apoptosis of U937 cell line. Methods Different dialysates were added into culture fluid with U937 cell line at exponential phase of growth, and groups were divided: fluid A+fluid B group (dialysate A+dialysate B), fluid A+fluid B+rottlerin (PKC? specific inhibitor)group, fluid A+powder B group (dialysate A+powder B) and fluid A+powder B + rottlerin group. Besides, blank control group and normal control group were established. Cells were harvested 24 h and 48 h after treatment, morphological changes were observed by Hoechst33258 fluorescence staining, cell apoptosis was measured by Annexin-V-FITC/PI double staining, and expression of PKC? mRNA and protein was detected by RT-PCR and Western blotting, respectively. Results Cell apoptosis significantly increased in fluid A+powder B group, with typical morphology of apoptosis. After treatment for 24 h and 48 h, cell apoptosis rates in fluid A+powder B group were significantly higher than those at corresponding time points in blank control group, normal control group and fluid A+powder B+rottlerin group (P0.05). Conclusion Fluid A+powder B can significantly increase apoptosis of U937 cell line, the mechanism of which may be associated with the up-regulation of expression of PKC?. Compared with fluid A+powder B, fluid A+fluid B is superior in reducing apoptosis of peripheral blood monouclear cells.
