Effects of methylprednisolone on expression of MMP-9 and airway inflammation in murine models of asthma
- VernacularTitle:甲泼尼龙对哮喘模型MMP-9的表达及对气道炎症的影响
- Author:
jian, ZHANG
;
jian-ming, ZHU
;
jian-wen, BAI
;
min-jia, LIN
;
shu-min, XU
- Publication Type:Journal Article
- Keywords:
asthma;
matrix metalloproteinase-9;
methylprednisolone
- From:Journal of Shanghai Jiaotong University(Medical Science)
2006;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of methylprednisolone(MP) on the expression of matrix metalloproteinase(MMP)-9 and airway inflammation in murine models of asthma. Methods Thirty female BALB/c mice were randomly divided into asthma group,MP group and control group(n=10).Murine models of acute asthma were established by ovalbumin(OVA) via peritoneal injection and intranasal instillation.The pathological changes of lung tissues were observed with HE staining,and cell quantitation was conducted in bronchalveolar lavage fluid(BALF).The expression of MMP-9 protein was determined by immunohistochemistry and gelatin zymogram,and the expression of MMP-9 mRNA was detected by RT-PCR. Results Compared with control group,there were more significant airway spasm and more infiltration of inflammatory cells in histologic examination,and there was higher eosinophil cell quantitation in BALF in asthma group(P
