Effects of hyperglycemia on proliferation,secretion function and expression of endoplasmic reticulum stress related molecules of pancreatic ? cell line
- VernacularTitle:高糖对胰岛?细胞增殖活力、分泌功能及内质网应激相关分子表达的影响
- Author:
hai-yan, ZHAO
;
lei, QIAN
;
xue-lian, FU
;
fan, LIN
;
hong-li, ZHANG
;
xiao, WANG
;
guo, LI
;
min, LUO
- Publication Type:Journal Article
- Keywords:
chronic hyperglycemia;
endoplasmic reticulum stress;
proliferation;
secreting function;
pancreatic ? cell
- From:Journal of Shanghai Jiaotong University(Medical Science)
2006;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of chronic hyperglycemia on the proliferation,secretion function and expression of endoplasmic reticulum stress(ERS) related molecules in pancreatic ? cell line MIN6. Methods MIN6 cells were treated with different concentrations of glucose(5.6,25 and 33.3mmol/L) and were harvested at indicated time(24h and 96h) for examinations.Cell proliferation was tested using CCK-8 solution,insulin and proinsulin secretion function was determined by ELISA,and mRNA expression of ERS related molecules(Total XBP-1,Spliced XBP-1) and protein expression of phosphorylated IRE1? were detected by Real-time PCR and Western blotting,respectively. Results After treatment with hyperglycemia for 96h, cell proliferation was significantly lower than that treated for 24h(P
